At first the College was sited in a little house on what is known today as Via del Tritone, opposite the church of Santa Maria in Constantinople.
In 1604 it was transferred to Via Felice, now called Via delle Quattro Fontane, and there it remained until 1962. During its time on the site opposite Palazzo Barberini the College went through several phases of building. The first united the mixture of houses into one combined building behind the facade that remains today. In the early part of the 20th century, the College was extended with a wing looking on to Via Rasella.
A National Church for Scotland was built in 1642. The Church of St Andrew of the Scots (Sant’Andrea degli Scozzesi) was built beside the College. Although the original Church still stands, it has since been deconsecrated.
The present College building on the Via Cassia was opened in 1964.